Truncated-gene reporter system for studying the regulation of manganese peroxidase expression.
Identifieur interne : 000B93 ( Main/Exploration ); précédent : 000B92; suivant : 000B94Truncated-gene reporter system for studying the regulation of manganese peroxidase expression.
Auteurs : J M Gettemy [États-Unis] ; D. Li ; M. Alic ; M H GoldSource :
- Current genetics [ 0172-8083 ] ; 1997.
Descripteurs français
- KwdFr :
- Activation enzymatique (MeSH), Azote (métabolisme), Basidiomycota (génétique), Basidiomycota (métabolisme), Gènes rapporteurs (MeSH), Isoenzymes (MeSH), Manganèse (métabolisme), Manganèse (pharmacologie), Peroxidases (effets des médicaments et des substances chimiques), Peroxidases (génétique), Peroxidases (métabolisme), Peroxyde d'hydrogène (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Régulation de l'expression des gènes fongiques (MeSH), Sulfure d'hydrogène (métabolisme), Température élevée (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Peroxidases.
- génétique : Basidiomycota, Peroxidases, Protéines recombinantes.
- métabolisme : Azote, Basidiomycota, Manganèse, Peroxidases, Peroxyde d'hydrogène, Protéines recombinantes, Sulfure d'hydrogène.
- pharmacologie : Manganèse.
- Activation enzymatique, Gènes rapporteurs, Isoenzymes, Régulation de l'expression des gènes fongiques, Température élevée.
English descriptors
- KwdEn :
- Basidiomycota (genetics), Basidiomycota (metabolism), Enzyme Activation (MeSH), Gene Expression Regulation, Fungal (MeSH), Genes, Reporter (MeSH), Hot Temperature (MeSH), Hydrogen Peroxide (metabolism), Hydrogen Sulfide (metabolism), Isoenzymes (MeSH), Manganese (metabolism), Manganese (pharmacology), Nitrogen (metabolism), Peroxidases (drug effects), Peroxidases (genetics), Peroxidases (metabolism), Recombinant Proteins (genetics), Recombinant Proteins (metabolism).
- MESH :
- chemical , drug effects : Peroxidases.
- chemical , genetics : Peroxidases, Recombinant Proteins.
- chemical , metabolism : Hydrogen Peroxide, Hydrogen Sulfide, Manganese, Nitrogen, Peroxidases, Recombinant Proteins.
- genetics : Basidiomycota.
- metabolism : Basidiomycota.
- chemical , pharmacology : Manganese.
- Enzyme Activation, Gene Expression Regulation, Fungal, Genes, Reporter, Hot Temperature, Isoenzymes.
Abstract
The expression of manganese peroxidase (MnP) in nitrogen-limited cultures of Phanerochaete chrysosporium is regulated by Mn, heat shock (HS), and H2O2 at the level of gene transcription. We have constructed a homologous gene reporter system to further examine the regulation of two mnp genes, mnp1 and mnp2, encoding individual MnP isozymes. Internal deletions of 234 and 359 bp were made within the coding regions of the mnp1 and mnp2 genes, respectively. The truncated mnp genes were subcloned into the shuttle vector pOGI18, which includes the Schizophylum commune ade5 gene as a selectable marker, and transformed into a P. chrysosporium Ade1 auxotrophic mutant. Northern-blot analysis of purified Ade+ transformants demonstrated that both of the truncated mnp genes were regulated in a manner similar to the endogenous mnp genes with respect to nitrogen limitation and induction by Mn, HS, and H2O2.
DOI: 10.1007/s002940050239
PubMed: 9211796
Affiliations:
Links toward previous steps (curation, corpus...)
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Alic</name></author><author><name sortKey="Gold, M H" sort="Gold, M H" uniqKey="Gold M" first="M H" last="Gold">M H Gold</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:9211796</idno><idno type="pmid">9211796</idno><idno type="doi">10.1007/s002940050239</idno><idno type="wicri:Area/Main/Corpus">000C07</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000C07</idno><idno type="wicri:Area/Main/Curation">000C07</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000C07</idno><idno type="wicri:Area/Main/Exploration">000C07</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Truncated-gene reporter system for studying the regulation of manganese peroxidase expression.</title><author><name sortKey="Gettemy, J M" sort="Gettemy, J M" uniqKey="Gettemy J" first="J M" last="Gettemy">J M Gettemy</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, P.O. Box 91000, Portland, OR 97291-1000, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, P.O. Box 91000, Portland, OR 97291-1000</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Li, D" sort="Li, D" uniqKey="Li D" first="D" last="Li">D. Li</name></author><author><name sortKey="Alic, M" sort="Alic, M" uniqKey="Alic M" first="M" last="Alic">M. Alic</name></author><author><name sortKey="Gold, M H" sort="Gold, M H" uniqKey="Gold M" first="M H" last="Gold">M H Gold</name></author></analytic><series><title level="j">Current genetics</title><idno type="ISSN">0172-8083</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term><term>Basidiomycota (metabolism)</term><term>Enzyme Activation (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Hot Temperature (MeSH)</term><term>Hydrogen Peroxide (metabolism)</term><term>Hydrogen Sulfide (metabolism)</term><term>Isoenzymes (MeSH)</term><term>Manganese (metabolism)</term><term>Manganese (pharmacology)</term><term>Nitrogen (metabolism)</term><term>Peroxidases (drug effects)</term><term>Peroxidases (genetics)</term><term>Peroxidases (metabolism)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation enzymatique (MeSH)</term><term>Azote (métabolisme)</term><term>Basidiomycota (génétique)</term><term>Basidiomycota (métabolisme)</term><term>Gènes rapporteurs (MeSH)</term><term>Isoenzymes (MeSH)</term><term>Manganèse (métabolisme)</term><term>Manganèse (pharmacologie)</term><term>Peroxidases (effets des médicaments et des substances chimiques)</term><term>Peroxidases (génétique)</term><term>Peroxidases (métabolisme)</term><term>Peroxyde d'hydrogène (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Sulfure d'hydrogène (métabolisme)</term><term>Température élevée (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peroxidases</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hydrogen Peroxide</term><term>Hydrogen Sulfide</term><term>Manganese</term><term>Nitrogen</term><term>Peroxidases</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term><term>Peroxidases</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term><term>Basidiomycota</term><term>Manganèse</term><term>Peroxidases</term><term>Peroxyde d'hydrogène</term><term>Protéines recombinantes</term><term>Sulfure d'hydrogène</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Manganèse</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Manganese</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Enzyme Activation</term><term>Gene Expression Regulation, Fungal</term><term>Genes, Reporter</term><term>Hot Temperature</term><term>Isoenzymes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation enzymatique</term><term>Gènes rapporteurs</term><term>Isoenzymes</term><term>Régulation de l'expression des gènes fongiques</term><term>Température élevée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The expression of manganese peroxidase (MnP) in nitrogen-limited cultures of Phanerochaete chrysosporium is regulated by Mn, heat shock (HS), and H2O2 at the level of gene transcription. We have constructed a homologous gene reporter system to further examine the regulation of two mnp genes, mnp1 and mnp2, encoding individual MnP isozymes. Internal deletions of 234 and 359 bp were made within the coding regions of the mnp1 and mnp2 genes, respectively. The truncated mnp genes were subcloned into the shuttle vector pOGI18, which includes the Schizophylum commune ade5 gene as a selectable marker, and transformed into a P. chrysosporium Ade1 auxotrophic mutant. Northern-blot analysis of purified Ade+ transformants demonstrated that both of the truncated mnp genes were regulated in a manner similar to the endogenous mnp genes with respect to nitrogen limitation and induction by Mn, HS, and H2O2.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9211796</PMID><DateCompleted><Year>1997</Year><Month>09</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0172-8083</ISSN><JournalIssue CitedMedium="Print"><Volume>31</Volume><Issue>6</Issue><PubDate><Year>1997</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Current genetics</Title><ISOAbbreviation>Curr Genet</ISOAbbreviation></Journal><ArticleTitle>Truncated-gene reporter system for studying the regulation of manganese peroxidase expression.</ArticleTitle><Pagination><MedlinePgn>519-24</MedlinePgn></Pagination><Abstract><AbstractText>The expression of manganese peroxidase (MnP) in nitrogen-limited cultures of Phanerochaete chrysosporium is regulated by Mn, heat shock (HS), and H2O2 at the level of gene transcription. We have constructed a homologous gene reporter system to further examine the regulation of two mnp genes, mnp1 and mnp2, encoding individual MnP isozymes. Internal deletions of 234 and 359 bp were made within the coding regions of the mnp1 and mnp2 genes, respectively. The truncated mnp genes were subcloned into the shuttle vector pOGI18, which includes the Schizophylum commune ade5 gene as a selectable marker, and transformed into a P. chrysosporium Ade1 auxotrophic mutant. Northern-blot analysis of purified Ade+ transformants demonstrated that both of the truncated mnp genes were regulated in a manner similar to the endogenous mnp genes with respect to nitrogen limitation and induction by Mn, HS, and H2O2.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gettemy</LastName><ForeName>J M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, P.O. Box 91000, Portland, OR 97291-1000, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Alic</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Gold</LastName><ForeName>M H</ForeName><Initials>MH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Curr Genet</MedlineTA><NlmUniqueID>8004904</NlmUniqueID><ISSNLinking>0172-8083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007527">Isoenzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>42Z2K6ZL8P</RegistryNumber><NameOfSubstance UI="D008345">Manganese</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical><Chemical><RegistryNumber>YY9FVM7NSN</RegistryNumber><NameOfSubstance UI="D006862">Hydrogen Sulfide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004789" MajorTopicYN="N">Enzyme Activation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="Y">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="Y">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006358" MajorTopicYN="N">Hot Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006862" MajorTopicYN="N">Hydrogen Sulfide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007527" MajorTopicYN="N">Isoenzymes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008345" MajorTopicYN="N">Manganese</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>6</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9211796</ArticleId><ArticleId IdType="doi">10.1007/s002940050239</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><noCountry><name sortKey="Alic, M" sort="Alic, M" uniqKey="Alic M" first="M" last="Alic">M. Alic</name><name sortKey="Gold, M H" sort="Gold, M H" uniqKey="Gold M" first="M H" last="Gold">M H Gold</name><name sortKey="Li, D" sort="Li, D" uniqKey="Li D" first="D" last="Li">D. Li</name></noCountry><country name="États-Unis"><region name="Oregon"><name sortKey="Gettemy, J M" sort="Gettemy, J M" uniqKey="Gettemy J" first="J M" last="Gettemy">J M Gettemy</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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